Spirogyra
Conventional brightfield image
Part of a Spirogyra, an aquatic plant.
QPI phase image
A QPI phase image of the Spirogyra. Darker areas are
optically thicker, meaning that light has taken longer to travel through those
areas. This could be because they are physically thicker, or because they are a different
material and have a different index of refraction.
QPI conventional dic image
Using both the QPI phase image and the brightfield image we are
able to emulate the optical processes that occur in the Differential Interference Contrast
optics within a microscope. This image contains both phase effects and intensity effects due
to the presence of the original brightfield data.
QPI pure dic image
By using only the QPI phase image we can emulate the effects
of DIC optics as though the sample were perfectly transparent, removing any intensity
effects from the image leaving only the remaining phase components. This is impossible with
an optical system, but straightforward with Iatia's computational phase imaging approach.