technology

Application notes

Our QPI technology has wide applicability across a range of disciplines and application areas. The application notes presented here demonstrate some of the ways that the QPI algorithm can be applied.

Optical microscopy

	Measurement of Red Blood Cell Volume Changes Researchers at the University of Melbourne's Physiology department utilised QPm phase images to measure red blood cell volume changes in response to osmotic stimuli.
	Measurement of Area Changes Researchers at the University of Melbourne's Pharmacology and Physiology departments utilised QPm phase images to measure the area of human airway smooth muscle cells, as a means of tracking growth rates over a period of time.
	Dual Imaging for Co-localisation QPI Phase Images are capable of showing morphological and physiological changes within samples as a supplementary technique or alternative to more time consuming and toxic fluorescence techniques.
	Automated Cell Counting Pure quantitative phase images, which measure changes in optical density throughout a sample, allow for improved contrast for thresholding purposes in automated cell counts when compared with a fluorescence image.
	Unstained Immunoperoxidase Labelled Paraffin Sections Phase Maps and Intensity-free modalities allow for the removal or inclusion of absorption effects of peroxidase which obscure underlying sample information.
	Identification of Cellular Structures QPI Phase Images provide quantitative phase and intensity data which may be used to identify cellular structures.
	Comparison with Optical Phase Contrast Modalities QPI digitally constructed phase contrast modalities compared with conventional optical phase contrast techniques.
	Intensity-Free Phase Imaging Intensity-free phase contrast modalities allow the removal of effects of highly absorptive structures in samples which may obscure underlying information.

Confocal microscopy

	QPm Application with Confocal Microscopes QPm and QPt are capable of generating the full suite of phase contrast modalities to aid in the co-localisation of fluorescence stain information without the need for additional phase contrast optics which may adversely affect the intensity of fluorescence information or cause a misalignment in the images captured.
	Study on Cat Retina Degeneration QPm and QPt are capable of generating phase contrast images for co-localisation purposes without the need for phase contrast optics or hardware.

Electron microscopy

	Recognition of Melanosomes QPe and QPt TEM Phase Contrast images assist in the recognition of melanosomes.
	Differential Interference Contrast for TEMs QPe and QPt DIC representations of samples aids in interpretation of complex structures.
	Magnetic Field Imaging QPe allows users to extract the magnitude and direction of the magnetic field surrounding the sample.

Forensics

	Magnetic Audio Tape QPm and QPt Phase Images provide clear surface topography information free of obscuring intensity data to allow confirmation of matches between recorded audio tapes and recording heads.
	Forged Cheques QPm and QPt Phase Images show clearer surface topography information providing additional valuable information for the matching of print to impact printers.
	Reconstruction of Defaced Engine Block Numbers QPm and QPt Phase Images show clearer surface topography information providing additional valuable information in the identification of ground engine block or serial numbers.
	Line Drawing Order QPm and QPt images are better able to provide surface topography and depth information free of obscuring intensity data (such as ink) to allow more accurate analysis of pen strokes.

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