Life sciences
Iatia's QPI provides a digital phase contrast solution in the life sciences above and beyond the capabilities of conventional lens-based solutions.
Segmentation
QPI provides an improved contrast mechanism enabling highly accurate cell segmentation. Unlike conventional phase contrast mechanisms, such as Differential Interference Contrast (DIC) or optical phase contrast, QPI provides quantitative phase data based on the optical thickness of the sample.
Optical phase contrast
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QPI
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Digital DIC
QPI is able to digitally generate Differential Interference Contrast (DIC) images from its phase and amplitude output. QPI DIC images are insensitive to birefringent plastic cultureware, unlike conventional DIC, and can be generated free of sample absorption which may obscure detail.
QPI also generates other phase contrast images such as "phase" (contrast) and Hoffman Modulation Contrast.
Optical DIC
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QPI DIC - absorption free
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Quantitative
QPI images quantitatively measure the optical thickness of the sample, making it suitable for cell volume determination/monitoring. Conventional phase imaging techniques, such as DIC do not provide this information.
Brightfield & QPI
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Line graph
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No specialized optics
QPI uses two defocused brightfield images captured with standard digital cameras making QPI practical where specialized phase optics and hardware are impractical or cumbersome.
High speed processing
QPI is a rapid solution supporting live capture of 640x480 pixel images at 15 frames per second.